Measurement of Lipid Hydroperoxides by the Ferric-Xylenol Orange Method (2) Application to Lipoxygenase Assay
نویسندگان
چکیده
منابع مشابه
Measurement of lipid hydroperoxides by the ferric-xylenol orange method (1) characteristics of the ferric-xylenol orange/membrane phosphatidylcholine complex.
The ferric-xylenol orange (FOX) method for measurement of hydroperoxides is based on a technique that employs reduction of peroxides in an acidic condition by Fe2+ and formation of the colored ferric-xylenol orange (XO/Fe3+) product with a peak at 560 nm. The 560 nm absorbance peak of XO/Fe3+ shifts to a 610 nm peak with high absorption intensity in the presence of phosphatidylcholine. This is ...
متن کاملDetermination of hydroperoxides in aqueous solutions containing surfactants by the ferrous oxidation-xylenol orange method.
Some surfactants widely used as additives in food, pharmaceuticals, and cosmetic formulations are susceptible to peroxidation resulting in accumulation of hydroperoxides (HP). Our investigation was aimed to study the possible influence of different surfactants on the proportionality and reproducibility of the ferrous oxidation-xylenol orange method developed originally for the determination of ...
متن کاملA Highly Sensitive Method for Measurement of Lipid Hydroperoxides by Iodimetry and Amperometric Endpoint.
T h i s report describes a procedure that permits accurate and specific measurement of lipid peroxides by iodimetry in the small samples available in many biological studies. Martin’s review (1) of methods for determination of organic peroxides indicates that most methods are based on reactions with various reducing substances. The most serious drawback of methods other than iodimetry can be as...
متن کاملMETHODS Method to Produce 9(S)-Hydroperoxides of Linoleic and Linolenic Acids by Maize Lipoxygenase
Seed from maize (corn) Zea mays provides a ready source of 9-lipoxygenase that oxidizes linoleic acid and Iinolenic acid into 9(5)-hydroperoxy-1 0(8, 12(Zl-octadecadienoic acid and 9(SJ-hydroperoxy-1 0(£), 12(Zj, 15(Zl-octadecatrienoic acid, respectively. Corn seed has a very active hydroperoxide-decomposing enzyme, allene oxide synthase (AOS), which must be removed prior to oxidizing the fatty...
متن کاملA Xylenol Orange-Based Screening Assay for the Substrate Specificity of Flavin-Dependent para-Phenol Oxidases.
Vanillyl alcohol oxidase (VAO) and eugenol oxidase (EUGO) are flavin-dependent enzymes that catalyse the oxidation of para-substituted phenols. This makes them potentially interesting biocatalysts for the conversion of lignin-derived aromatic monomers to value-added compounds. To facilitate their biocatalytic exploitation, it is important to develop methods by which variants of the enzymes can ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 2009
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.55.92